Question

Arrange the following steps in tissue culture in proper sequence:
A. Collection of explant,
B. Inoculation on medium,
C. Washing with detergent or Teepol,
D. Subculturing, E. Surface sterilization. Choose the correct answer from the options given below:

• A. A, C, E, B, D
• B. A, E, C, B, D
• C. C, A, D, E, B
• D. C, A, D, B, E

Hint:

In tissue culture, explant is collected, washed, sterilized, inoculated and then subcultured.

Answer 1

The Correct Option is A

Concept:
In tissue culture, contamination control is very important. Therefore, explant preparation follows a proper sequence.

Step 1: Collection of explant.
A suitable explant is first collected from the mother plant. \[ A=\text{Collection of explant} \]

Step 2: Washing.
The explant is washed with detergent or Teepol to remove surface dirt. \[ C=\text{Washing with detergent} \]

Step 3: Surface sterilization.
The explant is then treated with sterilizing chemicals. \[ E=\text{Surface sterilization} \]

Step 4: Inoculation.
The sterilized explant is placed on nutrient medium. \[ B=\text{Inoculation on medium} \]

Step 5: Subculturing.
After growth, the culture is transferred to fresh medium. \[ D=\text{Subculturing} \] Therefore, the correct sequence is: \[ A,C,E,B,D \] \[ \therefore \text{Correct Answer is (A)} \]