Question

Arrange the following in chromatography process order: A. Pack column with stationary phase, B. Inject sample (mobile phase), C. Partition/adsorption of analytes, D. Elution by mobile phase gradient, E. Detection and fraction collection.

• A. A, B, C, D, E
• B. C, B, A, D, E
• C. B, C, A, E, D
• D. D, A, C, E, B

Hint:

In chromatography, remember the sequence: prepare the column, load the sample, allow interaction, elute the components, and then detect or collect the fractions.

Answer 1

The Correct Option is A

Concept:
Chromatography is a separation technique used to separate components of a mixture. It works on the basis of differential interaction of analytes with the stationary phase and mobile phase.

Step 1: First prepare the column.
Before separation begins, the column must be packed with the stationary phase. \[ \text{First step} = A \]

Step 2: Introduce the sample.
After the column is prepared, the sample mixture is injected into the column along with the mobile phase. \[ \text{Second step} = B \]

Step 3: Interaction of analytes.
Different molecules interact differently with the stationary phase and mobile phase. This causes partitioning or adsorption of analytes. \[ \text{Third step} = C \]

Step 4: Elution process.
The mobile phase is passed through the column. Sometimes a gradient of mobile phase is used to elute different molecules at different times. \[ \text{Fourth step} = D \]

Step 5: Detection and collection.
Finally, the separated molecules are detected and collected as fractions. \[ \text{Fifth step} = E \] Therefore, the correct order is: \[ A, B, C, D, E \] \[ \therefore \text{Correct Answer is (A)} \]