List of top Plant Biotechnology Questions

Water is a liquid over the range of temperatures most compatible with life. The energy required to convert one mole of liquid water to one mole of water vapor is called
Exudation of sap from the cut surface of the stem of a well-watered herbaceous plant is due to the positive pressure in the xylem, which is termed as
Suspension culture is generally used for
Match List I with List II
List I (Vector):
A. pBR322
B. pUC8
C. pGEM3Z
D. M13mp1

List II (Description):
I. Vector facilitating in vitro transcription
II. Cloning vector based on bacteriophage
III. E. coli plasmid vector to clone DNA
IV. A lac selection plasmid
V. F plasmid based vector for handling longer DNA inserts for genomic library construction

Choose the correct answer from the options given below:
What is an open reading frame (ORF)?
The isoelectric point of a protein is defined as
Which of the following is an example of a protein domain?
Hot-start PCR involves the following steps
A. Adding PCR mixtures (water, buffer, dNTPs, primers, template)
B. Adding polymerase
C. Attaining reaction temperature
D. Continuing PCR to amplify the specific amplicons
What is the correct sequence in which these steps are performed?
Maximum number of types of alleles a recombinant inbred line population derived from crossing two genotypes of rice can generally show at an SSR locus is
Tissue culture for plant transformation should be free from
Agar, the gelling agent used for tissue culture media, is obtained from
A transgenic event is differentiated from other events by
Plasmid DNA isolation methods are primarily based on the
Function of ligase is
A) to repair single-stranded breaks that arise in double-stranded DNA
B) to join together two individual fragments of double stranded DNA
Choose the correct answer from the options given below:
Alkaline phosphatase is used to
Which of the restriction enzyme does produce fragments with blunt end upon restriction digestion?
Which of the following is NOT a millet crop?
What types of DNA molecules are copied using rolling circle replication process?
Genetic engineering involves the following steps
A. Gene manipulation and vector construction
B. Selection and confirmation of the transformants
C. Gene isolation
D. Genetic transformation
What is the correct sequence in which these steps are performed?
Plasmid vector will essentially have
Which of the following statement(s) are correct in describing the gene in DNA?
Statement I: The start/initiator codon for the genes can be found on the \(5^{\prime} \rightarrow 3^{\prime}\) strand of DNA
Statement II: The start/initiator codon for the genes can be found on the \(3^{\prime} \rightarrow 5^{\prime}\) strand of DNA
Choose the correct answer from the options given below: [Question ID = 656][Question Description = 156_34_PLB_AUG22_Q56]
Joining two DNA molecules using an adapter involves the following steps
A. Artificial synthesis of an adapter
B. Treating with polynucleotide kinase
C. Blunt end ligation to add an adapter to a blunt-ended DNA molecule
D. Joining two DNA molecules with sticky ends
What is the correct sequence in which these steps are performed? [Question ID = 658][Question Description = 158_34_PLB_AUG22_Q58]
Bt brinjal was developed for resistance to [Question ID = 659][Question Description = 159_34_PLB_AUG22_Q59]
Which of the following is also known as golden fibre? [Question ID = 660][Question Description = 160_34_PLB_AUG22_Q60]
Somatic embryogenesis is a desired method of plant regeneration for plant transformation because [Question ID = 657][Question Description = 157_34_PLB_AUG22_Q57]