List of top Plant Biotechnology Questions asked in ICAR AIEEA (PG)

Which of the following cannot be used for confirmation of positive clones?
Which of the following vector can be used for obtaining single-strand copies of a cloned sequence?

(1). pUC18
(2). Cosmid
(3). \( \lambda \) phage
(4). Phage M13
Which of the following is/are true for B-DNA?
(A). It is Right-handed
(B). It has 10.4 base pairs per turn of helix
(C). It's helix diameter is broadest among A and Z type
(D). The glycosidic bond is of anti type

Choose the correct answer from the options given below:
Which of the following can add the terminal 5'CCA3' at the end of mature tRNA?

(1). tRNA nucleotidyltransferase
(2). RNase D
(3). RNase P
(4). RNase E/F
What are Twintrons?

(1). Group II introns
(2). Group III introns
(3). Composite structure made up of two or more Group II and Group III introns
(4). An archaeal introns
Which of the following cellular structure found in all prokaryotes?
(A). Ribosome
(B). Capsules
(C). Flagellum

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Which of the following cellular structure is found only in a plant cells?

(1). Glyoxysomes
(2). Vacuoles
(3). Lysosomes
(4). Cytoskeleton
Which of the following restriction endonuclease requires \( \text{Mg}^{2+} \) for cleavage?

(1). EcoK
(2). EcoRI
(3). EcoB
(4). EcoP1
Match List-I with List-II:
Choose the correct answer from the options given below:
Which of the following phosphate is removed from the incoming nucleotide during the bacterial transcription?

(1). alpha
(2). gamma
(3). 5 prime
(4). 3 prime
Which of the following is not related with termination of transcription in E. coli?

(1). ppGpp
(2). pppGpp
(3). Alarmones
(4). pGp
Which of the following statement(s) is/are true about the role of Malonyl CoA in fatty acid metabolism?
(A). It is an inhibitor of the enzyme “Carnitine acyltransferase”
(B). High levels of malonyl CoA suppress fatty acid entry into the mitochondria
(C). High levels of malonyl CoA lead to reduced flux of fatty acids towards triglycerides' biosynthesis
(D). Low levels of malonyl CoA favour fatty acid oxidation

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Which of the following statement(s) is/are true about the Type IV isozyme of the hexokinase?
(A). It is highly specific for glucose and is thus often called as glucokinase.
(B). It has much higher \( K_m \) value for glucose
(C). It is allosterically inhibited by glucose-6-phosphate
(D). It is a non-inducible enzyme and follows Michaelis-Menten Kinetics

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Which of the following statements are true about the bundle sheath cells of \( C_4 \) plants?
(A). In comparison to mesophyll cells, they contain large number of agranal chloroplasts
(B). They have very thin cell wall, as compared to mesophyll cells, to facilitate gaseous exchange
(C). They have no intercellular spaces between them
(D). They generate less/no oxygen because of the low activity/lack of photosystem II

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Match List-I with List-II:
Choose the correct answer from the options given below:
Reading 5' \( \rightarrow \) 3', the sequence of following loops present in the clover leaf structure of t-RNA would be:
(A). Anticodon loop
(B). D-Loop
(C). T\( \psi \)C loop
(D). Variable loop
Choose the correct answer from the options given below:
Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A): The nitrogenase enzyme is considered to be a sluggish and inefficient enzyme.
Reason (R): The nitrogenase enzyme must go through several catalytic reduction cycles, wherein the two components viz. Fe-Protein and MoFe-Protein get dissociated from each other following each electron transfer, before final product i.e. ammonia appears.
In light of the above statements, choose the correct answer from the options given below:
The correct sequence for the flow of electrons between following components of mitochondrial electron transport chain is:
(A). NADH dehydrogenase
(B). Cytochrome \( bc_1 \) complex
(C). Ubiquinone
(D). Cytochrome C
Choose the correct answer from the options given below:
Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A): The citric acid cycle is amphibolic in nature rather than only catabolic.
Reason (R): Several biosynthetic pathways utilize citric acid cycle intermediates as precursors for the synthesis of important products.
In light of the above statements, choose the correct answer from the options given below:
Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A): In the early part of the twentieth century, 2,4-Dinitrophenol (DNP) was prescribed as a “diet pill” for weight loss.
Reason (R): The DNP reduces metabolic rate by uncoupling oxidative phosphorylation from electron transport.
In light of the above statements, choose the correct answer from the options given below:
Fructans are polymers of fructose built upon which one of the following starter units?
Which of the following enzymes contain an unusual amino acid viz. selenocysteine, at its active site?
The sequence for the action of the following enzymes of fatty acid \(\beta\)-oxidation pathway is:
(A). \(\beta\)-hydroxyacyl-CoA dehydrogenase
(B). Thiolase
(C). Enoyl CoA hydratase
(D). Acyl CoA - dehydrogenase
Choose the correct answer from the options given below:
When two phosphatidyl glycerol moieties join with each other with the elimination of one glycerol molecule, the resulting phospholipid formed is:
Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A) : In the secondary structure of proteins, the parallel \(\beta\)-pleated sheets are less stable than antiparallel \(\beta\)-pleated sheets.
Reason (R) : The hydrogen bonds of parallel \(\beta\)-pleated sheets are distorted in comparison to those of antiparallel \(\beta\)-pleated sheets.
In light of the above statements, choose the correct answer from the options given below: