List of top Plant Biotechnology Questions asked in ICAR AIEEA (PG)

Which of the following scorable marker fluoresces bright green color
The term "heterosis" was first used by
Which of the following is true for 35S promoter
Large fragment of DNA (chromosome) is separated by
Which of the following enzyme is used in second strand cDNA synthesis
Which of the following formulae may be used to estimate the concentration of RNA by UV spectrophotometer
Which of the following step of Polymerase Chain Reaction cycle operates at highest temperature
Which of the following DNA sequencing method uses dideoxyadenosine triphosphate?
A protein coding gene of an organism with unknown genome sequence can be isolated from
Conventionally the -10 ($5'$-TATAAT) and -35 ($5'$-TTGACA) regions are denoted in
Which of the following condition will favour synthesis of $\beta$-galactosidase in E. coli cells
Which of the following enzyme finally transfers ubiquitin to the target protein
Clonal propagation should not be used for the multiplication of following species
Which of the following will be present in the highest amount in the plant tissue culture media
Which of the following cannot be the form of a plant tissue culture method
Match List-I with List-II

List-I (Protein) & List-II (Feature)
(A). $\alpha$-Keratin & (II). Rich in Cysteine residues
(B). Collagen & (III). Rich in Glycine, Proline and Hydroxyproline
(C). Myoglobin & (I). Contains predominantly $\alpha$-helices in its secondary structure
(D). Concanavalin A & (IV). Contains $\beta$-pleated sheets in high abundance

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The DNA of the microorganisms living under conditions of extreme temperature is predominantly:
Fishermen, in certain parts of the world follow a practice of beating the roots of tree along riverbanks in a manner that transfers the extract of the roots into water. This practice paralyzes fishes and makes them easy prey. The most acceptable reason for this observation is:
Match List-I with List-II

List-I (Form of DNA) & List-II (Characteristic of DNA)
(A). A-DNA & (II). Short, broad and right-handed
(B). B-DNA & (III). Longer, thinner and right-handed
(C). Z-DNA & (IV). Anti conformation at Cytosine and syn at Guanine
(D). H-DNA & (I). Triplex DNA

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The products of which of the following pair of nif genes control the expression of other nif genes?
The order of elution (first to last) of amino acids Histidine (H), Glutamic acid (E), and Lysine (K) from a cation exchange column with a buffer having pH 6.0 is (Given: The pK values of H, E, and K is 6.0, 4.25, and 10.5 respectively):
The enzyme glutamine oxoglutarate amino transferase (GOGAT) is also known as
The sequence of action of following enzymes of plant fatty acid synthase complex is:
(A). $\beta$-Ketoacyl-ACP reductase
(B). $\beta$-Ketoacyl-ACP synthase
(C). $\beta$-Hydroxyacyl-ACP dehydratase
(D). Enoyl-ACP reductase
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Acrylamide, a toxic and potentially carcinogenic compound, can be formed in high heated starchy food products such as potato chips by which of the following precursor molecules?
Rubisco activase is a (an)

(A). Catalytic chaperone
(B). ATP-dependant enzyme
(C). Enzyme that activates Rubisco by promoting the entry of sugar phosphates to its active site
(D). Enzyme that activates Rubisco by increasing its carbamylation

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