List of top Animal Biotechnology Questions asked in ICAR AIEEA (PG)

Which of the following parameters affect the annealing of primer to the template in PCR?
(A) Nucleotide sequence of the primer.
(B) Temperature of annealing.
(C) Specificity of Taq DNA Polymerase.
(D) GC content of the template.
(E) Concentration of $\text{Mg}^{2+}$ ions.
Choose the correct answer from the options given below:
Which RNA molecule with the following feature used for cDNA synthesis?
Which of the following methods are used to label the double stranded DNA?
(A) 5' - end labelling with polynucleotide kinase
(B) 3' - end labelling with polynucleotide kinase
(C) 5' - end labelling with terminal transferase
(D) 3' - end labelling with terminal transferase
Choose the correct answer from the options given below:
Identify the order of post-trancriptional processing of yeast tRNA$^{\text{Tyr}$
(A) Addition of -CCA sequence at 3' end of tRNA
(B) Modification of Uridine to Dihydrouridine
(C) Removal of leader sequence from 5' end of tRNA
(D) Self splicing for removal intervening sequence
Choose the correct answer from the options given below:
Gunaosine, which is a leading nucleotide of the 'CAP' of mRNA is methylated at _____ position.
Which of the following antibiotic specifically inhibits the initiation of transcription in prokaryotes?
(A) Rifampicin
(B) Azithromycin
(C) Rifamycin B
(D) Cordycepin
Choose the correct answer from the options given below:
The inhibition of RNA synthesis in prokaryotes by the action of Actinomycin D emphasis the importance of which of following process?
Match List-I with List-II
List-I (Name of the enzyme in Eukaryotes) vs List-II (Type of RNA synthesized)
(A) RNA Pol I - (I) mRNA
(B) RNA Pol II - (II) tRNA
(C) RNA Pol III - (III) rRNA
Choose the correct answer from the options given below:
In Catabolite repression model of gene regulation, which of the following compound signals the absence of metabolite of choice for the energy to regulate the gene expression?
Which of the following process is regulated by the Trp operon?
The splice junction of eukaryotic is defined by which of the following sequence?
Match List-I with List-II
List-I (Phase of the Cell Cycle) vs List-II (Time Duration)
(A) M Phase - (I) $\sim$ 10 hours
(B) G1 Phase - (II) $\sim$ 1 hour
(C) S Phase - (III) 2 to 6 hours
(D) G2 Phase - (IV) 6 to 8 hours
Choose the correct answer from the options given below:
In E.coli, the enzymatic product of dnaG gene required for the synthesis of ________.
Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A) : Free energy requirment for DNA ligase enzyme is obtained in a species dependant manner.
Reason (R) : In E.coli, DNA ligase enzyme utilizes ATP and catalyzes the sealing of single strand nicks.
In light of the above statements, choose the correct answer from the options given below.
Which of the following enzyme is associated with the mitochondrial DNA synthesis?
Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A) : The use of RNA primer eliminates the source of error during replication.
Reason (R) : RNA is eventually replaced by DNA that permits actual base pairing to be achieved.
In light of the above statements, choose the correct answer from the options given below.
Which of the following function of the RNA Polymerase enzyme is affected when $\beta'$ subunit is mutated?
Given below are two statements, one is labeled as Assertion (A) and other one labeled as Reason (R).
Assertion (A) : The genetic code is not universal.
Reason (R) : In mammalian mitochondria, the codon 'UGA' specifies the amino acid 'Trp'
In light of the above statements, choose the correct answer from the options given below.
The antibiotic 'Puromycin' is structural analog of which of the following amino acyl tRNA?
Which of the following statements are TRUE with regard to the process of post-tranlational modifications?
(A) Cleavage of polyprotein to their component protein.
(B) Hydroxylation of praline/lysine amino acid residues of collagen protein.
(C) Formylation of 'Met' amino acid residues.
(D) Formation of disulphide bridges.
Choose the correct answer from the options given below:
Which of the following functions are associated with the DNA polymerase I
(A) Proof Reading
(B) Removal of Primer
(C) Nick Translation
(D) DNA Ligation
Choose the correct answer from the options given below:
Which of the following is explained by the Reiji Okazaki experimentation?
Among the following find the polypeptides synthesized non-ribosomally.
(A) Oxytocin
(B) Gramicidin S
(C) Insulin
(D) Actinomycin D
Choose the correct answer from the options given below:
Which of the following group of codons are synonymus in nature?
Folate is required for the normal DNA synthesis. Folate deficiency impairs the activity of the folate requiring enzyme synthase and thus the synthesis of which nucleotide is decreased.