List of top Questions asked in ICAR AIEEA (PG)- 2022

If a splice site were mutated so that splicing did not take place, what would be the effect on the mRNA?

First NIH guidelines for the conduct of recombinant DNA research were issued in which year?

The 4-amino or 4-keto group of pyrimidine bases is located in the

A DNA strand with sequence 5' CGATTC 3' would be complementary to the sequence

Which of the following describes the mode of action of Tetracycline as an antibiotic?

A 2 kb DNA fragment was cloned into BamHI and EcoRI sites located on a 8 kb cloning vector. The BamHI and EcoRI sites are adjacent to each other on the vector backbone. The vector contains an XhoI site located 300 bp upstream of the BamHI site. An internal XhoI site is present in the gene sequence. The resultant recombinant plasmid is digested with EcoRI and XhoI and analyzed through 1% agarose gel electrophoresis. Assuming complete digestion with EcoRI and XhoI, the DNA fragments (in base pairs) visible on the agarose gel will correspond to:

The polymerization of the gel used in polyacrylamide gel electrophoresis occurs between acrylamide and

The most plausible explanation for a sudden increase of the respiratory quotient (RQ) of a microbial culture is that

Reactions between antibodies and antigens that are detected by precipitate formation in an agar gel are referred as

Antibody response to a protein vaccine can only be obtained

Caenorhabditis elegans has a genome size of 103Mb, how many number of genes (approx.) are there in its genome?

Under certain circumstances, an elongating RNA polymerase can become arrested and cease transcribing. To deal with this situation, the cell has machinery that removes the arrested polymerase and at the same time recruits repair enzymes. Name the enzyme(s) which is part of the machinery.

The serum in mammalian cell culture medium stimulates cell growth and attachment. However, it must be filter sterilized prior to use to

Which of the following statement is correct about DNA vaccines?

Recently, it was reported that adult, differentiated cells can be made to behave as pluripotent stem cells by the introduction of a few genes, one of which was Oct What was the rationale for this experiment?

PCR of G-C rich regions (GC content $>$60%) pose some of the greatest challenges to PCR. However, this problem can be overcome by using which of the following?

Human Genome Project was officially initiated in which year and completed in which year?

Identify the correct order of events when epinephrine binds to \(\beta\)-receptor of the biological membrane.
A. Binding of GTP and release of GDP from \(\alpha\)-subunit of G-Protein.
B. Interaction of hormone bound \(\beta\)-receptor with \(\beta\gamma\) - subunit of G-Protein.
C. Binding of GTP bound \(\alpha\)-subunit of G-protein with Adenyl cyclase
D. Activation of dephosphorylation
E. Activation of phosphorylation
F. Activation of formation of cAMP
Choose the correct answer from the options given below:

Which of the following hormones are amino acid derivatives?
A. Epinephrine
B. Serotonin
C. GABA
D. Thyroxine
E. Vasopressin
Choose the correct answer from the options given below:

Match the following toxic chemicals with the suitable conjugants for the process of detoxification.
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Hershey and Chase designed an experiment using radioactive isotopes of sulphur and phosphorus to keep separate track of the viral proteins and nucleic acids during the infection process. Which bacteriophage and bacterium were used for this experiment?

Caesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, \(\rho\), of a double stranded Cs+DNA is given by the equation \(\rho = 66 + 0.098 X_{G+C}\), where \(X_{G+C}\) denotes

Which of the following causes decrease in albumin/globulin (A/G) ratio?
A. Glomerular Nephritis
B. Gastro-intestinal parasitism
C. Exocrine Pancreatic Insufficiency
D. Acute Inflammation
E. Pregnancy toxemia
F. Congestive heart failure
Choose the correct answer from the options given below:

Which of the following enzyme is considered to be liver specific in the case of dogs and cats, but not clinically significant in horses and ruminants?

In Protein Kinase A, the letter ‘A’ refers to