Question:
Why is ultrafiltration - method preferred over precipitation for concentrating proteins
Why is ultrafiltration - method preferred over precipitation for concentrating proteins
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Ultrafiltration = Physical pressure; Precipitation = Chemical additive.
Updated On: May 25, 2026
- It is only for small proteins
- Avoids chemical additives
- Denatures protein
- Removes endotoxin
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The Correct Option is B
Solution and Explanation
Step 1: Concept
Protein concentration is a key step in biotechnology to increase the potency of biological drugs.
Step 2: Meaning
Precipitation often uses salts or solvents to force proteins out of solution, which must then be removed.
Step 3: Analysis
Ultrafiltration uses semi-permeable membranes to separate proteins by size through pressure. It is a physical process that does not require adding chemicals that could harm the protein or require further purification.
Step 4: Conclusion
Ultrafiltration is preferred because it maintains the native state of the protein without introducing chemical contaminants. Final Answer: (B)
Protein concentration is a key step in biotechnology to increase the potency of biological drugs.
Step 2: Meaning
Precipitation often uses salts or solvents to force proteins out of solution, which must then be removed.
Step 3: Analysis
Ultrafiltration uses semi-permeable membranes to separate proteins by size through pressure. It is a physical process that does not require adding chemicals that could harm the protein or require further purification.
Step 4: Conclusion
Ultrafiltration is preferred because it maintains the native state of the protein without introducing chemical contaminants. Final Answer: (B)
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