Step 1: Understanding the Concept:
The discovery of biological nitrogen fixation (BNF) was a major milestone in agricultural science, establishing how plants acquire atmospheric nitrogen through symbiotic relationships.
Step 2: Detailed Explanation:
In 1838, French agricultural chemist Jean-Baptiste Boussingault performed precise quantitative sand-culture experiments.
He observed that leguminous plants could accumulate and fix nitrogen from the air when grown in soil completely devoid of nitrogenous compounds.
In contrast, non-leguminous plants (like cereals) failed to grow under identical conditions unless provided with external nitrogen sources.
M. W. Beijerinck later isolated the symbiotic bacteria (Rhizobium) in 1888.
Step 3: Final Answer:
Thus, J. B. Boussingault was the first to observe that legumes can utilize atmospheric nitrogen.