Question:

Which types of enzyme immobilization method is carrier free:

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Cross-linking = Hand-in-hand (No carrier needed).
Updated On: May 15, 2026
  • Cross linking method of enzyme immobilization
  • Entrapment method of enzyme immobilization
  • Covalent on support techniques
  • Adsorption method of enzyme immobilization
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The Correct Option is A

Solution and Explanation


Step 1: Concept

Enzyme immobilization restricts the movement of enzymes to enhance stability and allow reuse.

Step 2: Meaning

"Carrier-free" means the enzymes are joined together without being attached to a large external support or matrix.

Step 3: Analysis

Adsorption and Covalent methods use a support (carrier). Entrapment uses a matrix. Cross-linking uses bifunctional reagents (like glutaraldehyde) to link enzymes directly to each other.

Step 4: Conclusion

Cross-linking creates a self-immobilized enzyme system that does not require an additional carrier. Final Answer: (A)
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