Step 1: Define processes affecting heterozygosity.
Inbreeding (I): Inbreeding occurs when closely related individuals mate. This increases homozygosity and reduces heterozygosity in the population.
Genetic drift (II): Genetic drift is a random process that leads to the fixation or loss of alleles, reducing heterozygosity over time, particularly in small populations.
Mutation (III): Mutation introduces new genetic variation, which typically increases heterozygosity rather than reducing it.
Random mating (IV): Random mating does not alter heterozygosity directly, as it maintains the Hardy–Weinberg equilibrium in the absence of other evolutionary forces.
Step 2: Evaluate the options.
Option I and II (A): Correct. Both inbreeding and genetic drift reduce heterozygosity in populations.
Option II and III (B): Incorrect. Genetic drift reduces heterozygosity, but mutation increases it.
Option I and III (C): Incorrect. Inbreeding reduces heterozygosity, but mutation increases it.
Option II and IV (D): Incorrect. Genetic drift reduces heterozygosity, but random mating does not alter it.