Question

Which one is the correct product of DNA dependent RNA polymerase to the given template?
3’TACATGGCAAATATCCATTCA5’

• A. 5’AUGUACCGUUUAUAGGUAAGU3’
• B. 5’AUGUAAAGUUUAUAGGUAAGU3’
• C. 5’AUGUACCGUUUAUAGGGAAGU3’
• D. 5’ATGTACCGTTTATAGGTAAGT3’

Answer 1

The Correct Option is A

To determine the correct product of DNA-dependent RNA polymerase, we need to perform transcription on the provided DNA template strand. The given DNA template is:
3’TACATGGCAAATATCCATTCA5’.
During transcription, complementary base pairing rules apply: adenine (A) pairs with uracil (U) in RNA (replacing thymine (T) in DNA), thymine (T) pairs with adenine (A), cytosine (C) pairs with guanine (G), and guanine (G) pairs with cytosine (C).
Following these base pairing rules, the mRNA sequence produced from the 3’ to 5’ DNA strand will be transcribed as follows:

DNA	-	T	A	C	A	T	G	G	C	A	A	A	T	A	T	C	C	A	T	T	C	A
RNA	-	A	U	G	U	A	C	C	G	U	U	U	A	U	A	G	G	U	A	A	G	U

The transcribed RNA sequence is: 5'AUGUACCGUUUAUAGGUAAGU3'.
Thus, the correct product of the DNA-dependent RNA polymerase for the given template is the sequence that matches the options given as 5'AUGUACCGUUUAUAGGUAAGU3'.

Answer 2

DNA-dependent RNA polymerase transcribes the template strand of DNA to RNA. In this case, the RNA sequence will be complementary to the given DNA template:

Given DNA template strand (3' → 5'): TACATGGCAAATATCCATTCA

Transcribed RNA strand (5' → 3'): AUGUACCGUUUAUAGGUAAGU

Thus, the correct answer is (1) 5'AUGUACCGUUUAUAGGUAAGU3'.