Which one is the correct product of DNA dependent RNA polymerase to the given template?
3’TACATGGCAAATATCCATTCA5’
3’TACATGGCAAATATCCATTCA5’
- 5’AUGUACCGUUUAUAGGUAAGU3’
- 5’AUGUAAAGUUUAUAGGUAAGU3’
- 5’AUGUACCGUUUAUAGGGAAGU3’
- 5’ATGTACCGTTTATAGGTAAGT3’
The Correct Option is A
Approach Solution - 1
3’TACATGGCAAATATCCATTCA5’.
During transcription, complementary base pairing rules apply: adenine (A) pairs with uracil (U) in RNA (replacing thymine (T) in DNA), thymine (T) pairs with adenine (A), cytosine (C) pairs with guanine (G), and guanine (G) pairs with cytosine (C).
Following these base pairing rules, the mRNA sequence produced from the 3’ to 5’ DNA strand will be transcribed as follows:
| DNA | - | T | A | C | A | T | G | G | C | A | A | A | T | A | T | C | C | A | T | T | C | A |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RNA | - | A | U | G | U | A | C | C | G | U | U | U | A | U | A | G | G | U | A | A | G | U |
Thus, the correct product of the DNA-dependent RNA polymerase for the given template is the sequence that matches the options given as 5'AUGUACCGUUUAUAGGUAAGU3'.
Approach Solution -2
DNA-dependent RNA polymerase transcribes the template strand of DNA to RNA. In this case, the RNA sequence will be complementary to the given DNA template:
Given DNA template strand (3' → 5'): TACATGGCAAATATCCATTCA
Transcribed RNA strand (5' → 3'): AUGUACCGUUUAUAGGUAAGU
Thus, the correct answer is (1) 5'AUGUACCGUUUAUAGGUAAGU3'.
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अनुलेखन (ट्रांसक्रिप्शन) इकाई के संदर्भ में निम्नलिखित में से कौन-से कथन सही हैं?
A. डीएनए में अनुलेखन इकाई के मुख्यतया तीन भाग : उन्नायक, संरचनात्मक जीन और समापक होते हैं।
B. कहा जाता है कि उन्नायक, संरचनात्मक जीन के 5'-सिरे की ओर स्थित होता है।
C. उन्नायक एक डीएनए अनुक्रम है जो आरएनए पॉलिमरेज के लिए बंधन स्थल प्रदान करता है।
D. उन्नायक टेम्पलेट और कूटलेखन रज्जुक को परिभाषित करता है।
E. समापक, कूटलेखन रज्जुक के 3'-सिरे की ओर स्थित होता है और यह अनुलेखन प्रक्रिया के समापन को परिभाषित करता है।
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