Question

Which of the following restriction sites are present in amp\(^{R}\) gene of plasmid pBR322? (and = all, only = only)

\[ \begin{aligned} \text{A.} \;& \text{Pvu I} \\ \text{B.} \;& \text{Pst I} \\ \text{C.} \;& \text{BamH I} \\ \text{D.} \;& \text{Sal I} \end{aligned} \]

• A. A and D only
• B. B and C only
• C. A and B only
• D. C and D only

Hint:

Important restriction sites in pBR322: \[ \boxed{ \begin{aligned} &\mathrm{amp}^R &&\rightarrow \text{Pvu I, Pst I} &\mathrm{tet}^R &&\rightarrow \text{BamHI, SalI, HindIII} \end{aligned} } \]

Answer 1

The Correct Option is C

Step 1: Recall restriction sites in pBR322. The \(\mathrm{amp}^R\) (ampicillin resistance) gene of pBR322 contains the restriction sites \[ \boxed{\text{Pvu I and Pst I}.} \] BamHI and SalI are present elsewhere in the plasmid.

Step 2: Identify the correct combination. Hence, the restriction sites present in the \(\mathrm{amp}^R\) gene are \[ \boxed{\text{Pvu I and Pst I}.} \]

Step 3: Choose the correct option. Therefore, the correct option is \[ \boxed{(C).} \]