Concept:
Flow cytometry is used to identify, compare, and quantify cell surface markers such as CD markers. It is widely used in hematological malignancies and immunology.
Step 1: Understand CD markers.
CD markers are cell surface proteins used to identify different immune cell populations.
Step 2: Recall the best method.
Flow cytometry uses fluorescent-tagged antibodies to detect and quantify CD markers on cells.
Step 3: Compare with other tests.
ELISA detects soluble proteins, Western blot detects proteins by size, and IHC detects markers in tissue sections. Flow cytometry is best for quantifying CD markers in cell populations.
Therefore, the correct answer is Flow cytometry.