The correct answer is:
Option 3: Single-stranded
Plasmids are small, circular, double-stranded DNA molecules that are distinct from chromosomal DNA. They are found in bacteria and can also be found in some eukaryotic organisms. Let’s review each of the options:
Circular structure: Plasmids are typically circular in structure, though some rare plasmids may be linear. This is a common feature of plasmids.
Transferable: Plasmids are often transferable between bacteria, especially via processes like conjugation, making them important in horizontal gene transfer. This is another key feature of plasmids.
Single-stranded: This is not a feature of most plasmids. Plasmids are typically double-stranded, with both strands involved in replication. Some plasmids (e.g., certain types of viral plasmids) can have single-stranded forms during their replication cycle, but this is not characteristic of all plasmids.
Independent replication: Plasmids replicate independently of the bacterial chromosome, which is a key feature that allows them to exist as separate entities in the cell.
Thus, single-stranded is not a general feature of plasmids. They are typically double-stranded.
Match sequencing methods with key feature. 
Match the technique with its application. 
Given below are two statements:
Statement I: Transfer RNAs and ribosomal RNA do not interact with mRNA.
Statement II: RNA interference (RNAi) takes place in all eukaryotic organisms as a method of cellular defence.
In the light of the above statements, choose the most appropriate answer from the options given below:
Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."
Among many, the two core techniques that entitled the birth of modern biotechnology are :