Question:
Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Show Hint
Gene cloning essentials: Cut (restriction enzymes), Paste (ligase), Copy (polymerase).
Updated On: Apr 21, 2026
- A and B only
- D and E only
- B and C only
- C and D only
Show Solution
Verified By Collegedunia
The Correct Option is D
Solution and Explanation
Gene cloning involves cutting DNA (restriction enzymes), joining DNA fragments (DNA ligase), and copying DNA (DNA polymerase). DNA mutase (which isn't a standard enzyme used in molecular biology) and DNA recombinase (while involved in natural recombination, not typically a required enzyme in standard gene cloning protocols using plasmid vectors) are not essential for this process. DNA polymerase is essential for replicating the cloned DNA within the host organism.
Was this answer helpful?
6
0
Top NEET Biology Questions
- In spite of interspecific competition in nature, which mechanism the competing species might have evolved for their survival?
- Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?
- Mutations in plant cells can be induced by :
- Chordae tendineae are found in
- Glycolysis takes place in
View More Questions
Top NEET Molecular Biology Questions
- Which of the following is the correct match?
- Which of the following are correct about cellular respiration?
A. Cellular respiration is the breaking of C-C bonds of complex organic molecules by oxidation.
B. The entire cellular respiration takes place in Mitochondria.
C. Fermentation takes place under anaerobic condition in germinating seeds.
D. The fate of pyruvate formed during glycolysis depends on the type of organism also.
E. Water is formed during respiration as a result of O2 accepting electrons and getting reduced
Choose the correct answer from the options given below: - Given below are two statements :
Statement I: In the lac operon, the z gene codes for beta-galactosidase which is primarily responsible for the hydrolysis of lactose into galactose and glucose.
Statement II: In addition to lactose, glucose or galactose can also induce lac operon.
In the light of the above statements, choose the correct answer from the options given below : - Which of the following are correct about EcoR1?
A. Cut the DNA with blunt end
B. Cut the DNA with sticky end
C. Recognise a specific palindromic sequence
D. Cut the DNA between the base G and A when encounters the DNA sequence ‘GAATTC’
E. Exonuclease
Choose the correct answer from the options given below: - Given below are two statements
Statement I : RNA interference takes place in all Eukaryotic organisms as method of cellular defense.
Statement II : RNAi involves the silencing of a specific mRNA due to a complementary single-stranded RNA molecule that binds and prevents translation of mRNA
In the light of the above statements, choose the correct answer from the options given below
View More Questions
Top NEET Questions
- In spite of interspecific competition in nature, which mechanism the competing species might have evolved for their survival?
- A coil of inductive reactance $31\, \Omega$ has a resistance of 8 $\Omega$. It is placed in series with a condenser of capacitative reactance $25 \,\Omega$. The combination is connected to an a.c. source of 110 V. The power factor of the circuit is :-
- A car starts from rest and accelerates at $5\, m / s ^{2}$ At $t=4\, s$, a ball is dropped out of a window by a person sitting in the car. What is the velocity and acceleration of the ball at $t =6\, s$ ? (Take $\left. g =10\, m / s ^{2}\right)$
- A liquid compound (x) can be purified by steam distillation only if it is
- In an a.c. circuit with phase voltage V and current I, the power dissipated is
View More Questions