Question

Which application is used to detect genetic disorders at the DNA level?

• A. Western blotting
• B. ELISA
• C. PCR
• D. Chromatography

Hint:

Important laboratory techniques: \[ \boxed{ \begin{aligned} \text{PCR} &\rightarrow \text{DNA amplification} \text{Western blot} &\rightarrow \text{Protein detection} \text{ELISA} &\rightarrow \text{Antigen/Antibody detection} \end{aligned} } \]

Answer 1

The Correct Option is C

Step 1: Recall the applications of the given techniques. \[ \begin{aligned} \text{Western blotting} &\rightarrow \text{Detection of proteins}, \text{ELISA} &\rightarrow \text{Detection of antigens or antibodies}, \text{PCR} &\rightarrow \text{Amplification and analysis of DNA}, \text{Chromatography} &\rightarrow \text{Separation of biomolecules}. \end{aligned} \]

Step 2: Identify the technique used for DNA diagnosis. Polymerase Chain Reaction (PCR) amplifies specific DNA sequences and is widely used to detect mutations responsible for genetic disorders. Hence, \[ \boxed{\text{PCR}} \] is the correct answer. Therefore, \[ \boxed{(C)} \] is the correct answer.