Question

The technique used to identify the specific DNA in bacterial colonies is:

• A. In-situ hybridization
• B. Dot blot
• C. Colony hybridization
• D. Western hybridization

Hint:

Review the techniques used for identifying specific DNA sequences in bacterial and other microbial colonies.

Answer 1

The Correct Option is C

Colony hybridization is a molecular biology technique used to detect specific DNA sequences within bacterial colonies. Colonies are transferred to a membrane, lysed to release DNA, and then hybridized with a labeled DNA or RNA probe that binds to the target sequence. This process enables the identification of colonies carrying a specific gene or sequence of interest.