Question:
Isoelectric focusing allows separation of the proteins according to their:
Isoelectric focusing allows separation of the proteins according to their:
Show Hint
Understand the concept of isoelectric point (pI) and its role in protein separation techniques.
Updated On: Feb 11, 2026
- pH
- pI
- pKa
- pKb
Show Solution
Verified By Collegedunia
The Correct Option is B
Solution and Explanation
Isoelectric focusing (IEF) separates proteins based on their isoelectric point (pI), which is the pH at which the protein has no net charge. Proteins migrate in a pH gradient during IEF until they reach the pH corresponding to their pI, where they cease to move as the net charge becomes zero.
Was this answer helpful?
0
0
Top CUET PG Life Science Questions
- The portion of transmembrane proteins that cross the lipid bilayer are usually:
- Match List I with List II:Choose the correct answer from the options given below:
List I (Enzyme) List II (Function) (A) Cytochrome oxidase (I) Electron transport system (B) Topoisomerases (II) Change linking number (C) Cohesins (III) DNA Replication (D) PCNA (IV) Cell cycle - Which of the following enzyme is inhibited by citrate in Kreb's cycle?
- Why does the presence of proline destabilize α-helix?
(A) Proline residue introduces a destabilizing kink in the α-helix
(B) In proline, the nitrogen atom is a part of a rigid ring, and rotation about N-C bond is not possible
(C) The nitrogen atom of a proline residue in a peptide linkage has no substituent hydrogen to participate in hydrogen bonds with other residues
(D) Proline residue works as a helix linker
Choose the correct answer from the options given below: - Which of the following are components of lipid rafts in plasma membrane?
(A) Cholesterol
(B) Sphingolipids
(C) Glycolipids
(D) Phospholipids
Choose the correct answer from the options given below:
View More Questions
Top CUET PG Techniques Questions
- The portion of transmembrane proteins that cross the lipid bilayer are usually:
- Which of the following is not a fluorescence dye?
- The technique used to identify the specific DNA in bacterial colonies is:
- Which metal is used for electron beam generation in scanning electron microscopy?
- What is the role of sodium dodecyl sulfate (SDS) in protein electrophoresis?
(A) Add net negative charge to the protein
(B) Separate lipids on the basis of their molecular weight
(C) Modify native conformation of a protein
(D) Allows direct destaining of the proteins
Choose the correct answer from the options given below:
View More Questions
Top CUET PG Questions
- Given below are two statements:
Statement I: For Plato the meaning of the word 'Justice' is basically a conception in the mind only.
Statement II: For Plato the meaning of the word 'Justice' is fixed, mind-independently
In the light of the above statements, choose the correct answer from the options given below: - What is the electron count of OS6CO182-?
- XeF4 hydrolysis gives A + B, HF, and Oxygen. Find A and B
- What is more basic in pyrol and pyridine?
- Hydrolysis of Al2C3, Be2?
View More Questions