Question:
Tetracycline resistance gene of PBR322 shows recognition site for which restriction enzyme?
Tetracycline resistance gene of PBR322 shows recognition site for which restriction enzyme?
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Know restriction enzyme sites in common vectors (PBR322, pUC).
EcoRI → TetR, BamHI → AmpR, HindIII → multiple cloning sites.
Useful for recombinant DNA experiments.
EcoRI → TetR, BamHI → AmpR, HindIII → multiple cloning sites.
Useful for recombinant DNA experiments.
Updated On: Mar 6, 2026
- BamHI
- EcoRI
- Pst I
- Hind III
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The Correct Option is B
Solution and Explanation
1. PBR322 plasmid carries AmpR and TetR genes.
2. EcoRI cuts specifically in the TetR gene for cloning.
3. Other enzymes cut at different sites.
4. Hence, correct answer is (2) EcoRI.
2. EcoRI cuts specifically in the TetR gene for cloning.
3. Other enzymes cut at different sites.
4. Hence, correct answer is (2) EcoRI.
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