Question

Tetracycline resistance gene of PBR322 shows recognition site for which restriction enzyme?

Hint:

Know restriction enzyme sites in common vectors (PBR322, pUC).
EcoRI → TetR, BamHI → AmpR, HindIII → multiple cloning sites.
Useful for recombinant DNA experiments.

Answer 1

1. PBR322 plasmid carries AmpR and TetR genes.
2. EcoRI cuts specifically in the TetR gene for cloning.
3. Other enzymes cut at different sites.
4. Hence, correct answer is (2) EcoRI.