Question

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tet^R) gene in the pBR322 cloning vector

• A. Bam HI and Sal I
• B. Sal I and Pst I
• C. Pst I and Pvu I
• D. Pvu I and Bam HI

Answer 1

The Correct Option is A

The pBR322 plasmid contains the tetracycline resistance (\( \text{tet}^+ \)) gene. The restriction sites for Bam HI and Sal I are located within the \( \text{tet}^+ \) gene. Cutting the plasmid with either of these enzymes will disrupt the \( \text{tet}^+ \) gene, allowing for the selection of recombinant plasmids (those that have taken up a foreign DNA insert).