Arranging the vaccines by historical order of invention:
- The Smallpox vaccine was the first vaccine ever invented by Edward Jenner in 1796, but the Anthrax vaccine (by Louis Pasteur) predates many modern vaccines and is considered one of the earliest bacterial vaccines (1881).
- So, Anthrax vaccine comes first.
- Then Smallpox vaccine (widely recognized historically for human use).
- Diphtheria Toxoid was developed in the 1920s.
- Yellow Fever Vaccine was developed in 1935.
- Hepatitis B Vaccine is more recent, first licensed in the 1980s.
So, the ascending order is: E (Anthrax), D (Smallpox), C (Diphtheria), B (Yellow Fever), A (Hepatitis B).