Question

Match the pigments given in List I with their colour in chromatogram given in List II.

List-I (Pigments)		List-II (Colour in chromatogram)
1	Chlorophyll 'b'	p	Yellow orange
2	Carotenoids	q	Orange red
3	Chlorophyll 'a'	r	Yellow
4	Xanthophylls	s	Blue green
		t	Yellow green

Choose the correct option from the following :

• A. 1-s, 2-t, 3-r, 4-q
• B. 1-t, 2-p, 3-s, 4-r
• C. 1-p, 2-q, 3-r, 4-t
• D. 1-t, 2-p, 3-r, 4-s

Hint:

Chromatography helps separate plant pigments based on their solubility and interaction with the solvent.

Answer 1

The Correct Option is B

Matching photosynthetic pigments with their chromatographic colors:

1. Chlorophyll 'b' - t. Yellow green: This accessory pigment appears yellow-green in chromatography.

2. Carotenoids - p. Yellow orange: These accessory pigments (including β-carotene) show bright yellow-orange hues.

3. Chlorophyll 'a' - s. Blue green: The primary photosynthetic pigment appears blue-green on chromatograms.

4. Xanthophylls - r. Yellow: These oxygenated carotenoids (e.g., lutein) appear distinctly yellow.

The correct matching is: (B) 1-t, 2-p, 3-s, 4-r

Other options are incorrect because: - (A) Mismatches all pigment colors - (B) Incorrectly assigns colors to chlorophylls - (D) Reverses chlorophyll 'a' and xanthophyll colors

Answer 2

Chlorophyll 'b': Appears yellow-green in a chromatogram.

Carotenoids: Appear orange-red in a chromatogram.

Chlorophyll 'a': Appears blue-green.

Xanthophylls: Appear yellow.

Option (B) is correct because it correctly matches the pigments with their chromatogram colors.