Question

Match the Following:

• A. A - I, B - IV, C - III, D - V, E - II
• B. A - III, B - II, C - IV, D - V, E - I
• C. A - III, B - I, C - IV, D - V, E - II
• D. A - IV, B - II, C - III, D - V, E - I

Hint:

Tyndallization is synonymous with fractional sterilization. Incineration is the standard method for sterilizing inoculation loops in the laboratory using a Bunsen burner.

Answer 1

The Correct Option is C

Step 1: Understanding the Concept:
This question matches physical and biological terms associated with pasteurization, sterilization, and decomposition with their respective methods, materials, or characteristics.

Step 2: Detailed Explanation:
Let us match each term in List I with its corresponding description in List II:
A. Pasteurization (III. Extends shelf life of Milk):
Pasteurization is a heat treatment process developed by Louis Pasteur that destroys pathogenic vegetative microorganisms in liquids (such as milk) without significantly altering nutrient content or taste, thereby extending shelf life.
B. Tyndallization (I. Fractional Sterilization):
Tyndallization is a fractional sterilization method that involves heating a material to \(100^{\circ}\text{C}\) for three consecutive days with incubation periods in between.
This process allows heat-resistant bacterial endospores to germinate into vulnerable vegetative cells, which are then killed during subsequent heating cycles.
C. Radiation sterilization (IV. Heat sensitive material):
Ionizing radiation (such as gamma rays or electron beams) is a form of cold sterilization used to sterilize heat-sensitive medical and laboratory items (such as plastic syringes, Petri dishes, and sutures) that would melt or degrade under steam heat.
D. Incineration (V. Inoculation loops and needles):
Incineration is a dry heat sterilization process that uses direct flame to burn organic matter to ash.
It is commonly used in microbiology laboratories to rapidly sterilize metal inoculating loops and needles.
E. Putrefaction (II. Foul smell due to anaerobic decomposition of proteins):
Putrefaction refers to the anaerobic decomposition of proteins by bacterial enzymes, which yields foul-smelling organic compounds like cadaverine, putrescine, and hydrogen sulfide.
This matching corresponds to: A-III, B-I, C-IV, D-V, E-II.

Step 3: Final Answer:
Hence, the correct matched sequence is A-III, B-I, C-IV, D-V, E-II.