Question

Match LIST-I with LIST-II 

LIST-I		LIST-II
A.	Gynogenesis	I.	Callus culture
B.	Culturing in liquid medium	II.	Ovary culture
C.	Androgenesis	III.	Suspension culture
D.	Culturing on agar medium	IV.	Pollen culture

Choose the correct answer from the options given below: 
 

• A. A-II, B-III, C-IV, D-I
• B. A-III, B-IV, C-II, D-I
• C. A-II, B-IV, C-I, D-III
• D. A-I, B-II, C-III, D-IV

Hint:

Use prefixes to remember the haploid production methods: {Gyno-} refers to the female part (ovary/ovule), while {Andro-} refers to the male part (anther/pollen). Also, associate liquid medium with suspension culture and solid (agar) medium with callus culture.

Answer 1

The Correct Option is A

Step 1: Understanding the Concept:
This question requires matching specific biological processes for haploid production and general tissue culture methods with their corresponding laboratory techniques.
Step 2: Detailed Explanation:
A. Gynogenesis: This is the development of a haploid plant from the female gametophyte (egg cell or other cells of the embryo sac). This is achieved in vitro through II. Ovary culture or ovule culture.
B. Culturing in liquid medium: When plant cells or small cell aggregates are grown in a constantly agitated liquid nutrient medium, it is known as a III. Suspension culture. This is used for producing single cells or secondary metabolites.
C. Androgenesis: This is the development of a haploid plant from the male gametophyte (microspore). The most common technique for this is IV. Pollen culture or anther culture.
D. Culturing on agar medium: When plant tissues are grown on a solid or semi-solid medium gelled with agar, it often leads to the formation of an unorganized mass of cells known as a callus. This is a form of I. Callus culture.
Step 3: Final Answer:
The correct matches are: A-II, B-III, C-IV, D-I.