Question

Match List I with List II

• A. A(II), B(IV), C(III), D(I)
• B. A(IV), B(II), C(III), D(I)
• C. A(II), B(III), C(I), D(IV)
• D. A(III), B(I), C(IV), D(II)

Hint:

Needle -> Red heat (Incineration).
Antibiotics -> Filter (Heat sensitive).
Media -> Autoclave (Moist heat).
Seeds -> Bleach (Surface sterilization).

Answer 1

The Correct Option is C

Step 1: Understanding the Question:
The task is to match specific laboratory sterilization or disinfection techniques with the items they are most commonly used to treat.

Step 2: Detailed Explanation:

• A. Incineration: This is a dry heat method where items are burned to ashes. In the lab, inoculating loops and needles are held in a flame until they glow red-hot to kill all microbes. Hence, A matches with II.

• B. Sodium hypochlorite: This is a chemical disinfectant (bleach). In plant pathology and tissue culture, seeds are often soaked in a dilute bleach solution to kill microbes on the surface without damaging the internal embryo. Hence, B matches with III.

• C. Moist heat sterilization: This usually refers to autoclaving ($121^{\circ}\text{C}$/15 psi). It is the standard method for sterilizing most bacteriological culture media. Hence, C matches with I.

• D. Filter sterilization: Heat-sensitive (thermo labile) liquids like antibiotics, vitamins, or certain enzymes would be destroyed by heat. They are sterilized by passing them through a membrane filter with a pore size of $0.22\ \mu m$. Hence, D matches with IV.

Step 3: Final Answer:
The matching sequence is A-II, B-III, C-I, D-IV. This corresponds to option (C).