Question:
Isolation of Chlamydia from a tissue specimen can be done by:
Isolation of Chlamydia from a tissue specimen can be done by:
Show Hint
Isolation means growing the organism; Chlamydia needs living cells like the egg yolk sac.
Updated On: Jun 24, 2026
- ELISA (enzyme linked immunosorbent assay)
- Yolk sac inoculation
- Direct immunofluorescence antibody test (DFA)
- Polymerase chain reaction (PCR)
Show Solution
Verified By Collegedunia
The Correct Option is B
Solution and Explanation
Step 1: The key word is isolation. Isolation means recovering the live organism by growing it, not just detecting its antigen or nucleic acid.
Step 2: Chlamydiae are obligate intracellular bacteria, so they cannot grow on ordinary cell free media. They need living cells. Classic methods are inoculation into the yolk sac of an embryonated hen egg or into cell cultures such as McCoy cells.
Step 3: Among the options, yolk sac inoculation is the only true culture or isolation method. It supports the intracellular growth cycle of Chlamydia.
Step 4: Why the others are wrong. ELISA detects chlamydial antigen, it is a detection test, not isolation. Direct immunofluorescence with antibody detects elementary bodies in a smear, again detection, not isolation. PCR detects chlamydial DNA, which is detection of nucleic acid and not recovery of a living organism.
Conclusion: Isolation is done by yolk sac inoculation, option B.
Step 2: Chlamydiae are obligate intracellular bacteria, so they cannot grow on ordinary cell free media. They need living cells. Classic methods are inoculation into the yolk sac of an embryonated hen egg or into cell cultures such as McCoy cells.
Step 3: Among the options, yolk sac inoculation is the only true culture or isolation method. It supports the intracellular growth cycle of Chlamydia.
Step 4: Why the others are wrong. ELISA detects chlamydial antigen, it is a detection test, not isolation. Direct immunofluorescence with antibody detects elementary bodies in a smear, again detection, not isolation. PCR detects chlamydial DNA, which is detection of nucleic acid and not recovery of a living organism.
Conclusion: Isolation is done by yolk sac inoculation, option B.
Was this answer helpful?
0
0
Top NEET PG Microbiology Questions
- A patient hailing from Delhi presents with fever, arthralgia, and extensive petechial rash for 3 days. Lab investigations revealed a hemoglobin of 9 g/ dL, a white blood cell count of 9000 cells/mm3, a platelet count of 20000 cells/mm3, and a prolonged bleeding time. The clotting time was normal. What is the most likely diagnosis?
- Which of the following statements is true about Trichomonas vaginalis?
- A lady from West Rajasthan presented with an ulcer surrounded by erythema on the right leg. Microscopy of the biopsy from the edge of the ulcer showed organisms with dark staining nuclei and kinetoplast. What is the most likely causative agent?
- A child presents with a fever and a rash. Urine examination showed cells with owl's eye appearance. What is the most likely diagnosis?
- Infection with Clonorchis sinensis is associated with an increased risk of__?
View More Questions
Top NEET PG Questions
- A patient hailing from Delhi presents with fever, arthralgia, and extensive petechial rash for 3 days. Lab investigations revealed a hemoglobin of 9 g/ dL, a white blood cell count of 9000 cells/mm3, a platelet count of 20000 cells/mm3, and a prolonged bleeding time. The clotting time was normal. What is the most likely diagnosis?
- Which of the following statements is true about Trichomonas vaginalis?
- A lady from West Rajasthan presented with an ulcer surrounded by erythema on the right leg. Microscopy of the biopsy from the edge of the ulcer showed organisms with dark staining nuclei and kinetoplast. What is the most likely causative agent?
- A child presents with a fever and a rash. Urine examination showed cells with owl's eye appearance. What is the most likely diagnosis?
- Infection with Clonorchis sinensis is associated with an increased risk of__?
View More Questions