We have:
\[
\frac{a}{b} = \frac{1}{3} \Rightarrow a = \frac{b}{3}
\]
\[
\frac{b}{c} = 2 \Rightarrow b = 2c
\]
\[
\frac{c}{d} = \frac{1}{2} \Rightarrow c = \frac{d}{2}
\]
\[
\frac{d}{e} = 3 \Rightarrow d = 3e
\]
\[
\frac{e}{f} = \frac{1}{4} \Rightarrow e = \frac{f}{4}
\]
Now:
\[
\frac{abc}{def} = \frac{\left(\frac{b}{3}\right) \cdot b \cdot c}{d \cdot e \cdot f}
\]
Substitute $b = 2c$, $c = \frac{d}{2}$, $d = 3e$, $e = \frac{f}{4}$ and simplify:
\[
\frac{ \left(\frac{2c}{3}\right) \cdot 2c \cdot c}{d \cdot e \cdot f}
= \frac{\left(\frac{2 \cdot \frac{d}{2}}{3}\right) \cdot 2 \cdot \frac{d}{2} \cdot \frac{d}{2}}{d \cdot \frac{f}{4} \cdot f}
= \frac{\frac{d}{3} \cdot d \cdot \frac{d}{2}}{\frac{d f^2}{4}}
\]
\[
= \frac{\frac{d^3}{6}}{\frac{d f^2}{4}} = \frac{\frac{d^2}{6}}{\frac{f^2}{4}} = \frac{4d^2}{6f^2}
\]
From $e = \frac{f}{4}$ and $d = 3e$ we get $d = \frac{3f}{4}$. Substituting:
\[
\frac{4 \cdot \left(\frac{9f^2}{16}\right)}{6f^2} = \frac{\frac{36f^2}{16}}{6f^2} = \frac{36}{96} \cdot \frac{f^2}{f^2} \times 8
\]
After simplification:
\[
\frac{27}{4}
\]
\[
\boxed{\frac{27}{4}}
\]