We are given
\[
5\sin\theta=4.
\]
Therefore,
\[
\sin\theta=\frac{4}{5}.
\]
Now,
\[
\cosec\theta=\frac{1}{\sin\theta}.
\]
So,
\[
\cosec\theta=\frac{5}{4}.
\]
Using the identity
\[
\sin^2\theta+\cos^2\theta=1,
\]
we get
\[
\left(\frac{4}{5}\right)^2+\cos^2\theta=1.
\]
\[
\frac{16}{25}+\cos^2\theta=1.
\]
\[
\cos^2\theta=\frac{9}{25}.
\]
Taking positive value,
\[
\cos\theta=\frac{3}{5}.
\]
Now,
\[
\cot\theta=\frac{\cos\theta}{\sin\theta}.
\]
\[
\cot\theta=\frac{\frac{3}{5}}{\frac{4}{5}}.
\]
\[
\cot\theta=\frac{3}{4}.
\]
Now substitute in the expression:
\[
\frac{\cosec\theta-\cot\theta}{\cosec\theta+\cot\theta}
=
\frac{\frac{5}{4}-\frac{3}{4}}{\frac{5}{4}+\frac{3}{4}}.
\]
\[
=
\frac{\frac{2}{4}}{\frac{8}{4}}.
\]
\[
=
\frac{2}{8}.
\]
\[
=
\frac{1}{4}.
\]
Hence, the required value is
\[
\frac{1}{4}.
\]