Question:
Identify A, B, C and D in the diagram of E.coli cloning vector of pBR322:
Identify A, B, C and D in the diagram of E.coli cloning vector of pBR322:
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Memorize common restriction sites on pBR322.
AmpR and TetR = selectable markers.
Ori = origin of replication.
AmpR and TetR = selectable markers.
Ori = origin of replication.
Updated On: Mar 6, 2026
- A-Hind I, B-ECoR1, C-ampR, D-Ori
- A-Hind I, B-BamHI, C-KanR, D-ampR
- A-BamHI, B-Pst I, C-Ori, D-ampR
- A-ECoR1, B-BamHI, C-ampR, D-Ori
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The Correct Option is A
Solution and Explanation
1. pBR322 map: Hind I → site A, EcoRI → site B, ampR → antibiotic resistance, Ori → origin of replication.
2. BamHI & KanR are different sites/genes.
3. Correct identification is (1) A-Hind I, B-ECoR1, C-ampR, D-Ori.
2. BamHI & KanR are different sites/genes.
3. Correct identification is (1) A-Hind I, B-ECoR1, C-ampR, D-Ori.
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