Question

Identify A, B, and C in the diagram of pBR322 (A, B, and C are restriction sites):

 

• A. BamH I, Hind III, Pst II
• B. BamH I, Hind III, Pst I
• C. Hind III, BamH I, Pst II
• D. Pst I, PVU II, BamH I

Hint:

Familiarizing yourself with restriction enzyme recognition sites and their locations on cloning vectors like pBR322 is essential for effective molecular biology experimentation.

Answer 1

The Correct Option is B

In the plasmid map of pBR322, the restriction sites are crucial for molecular cloning:
A: BamH I, recognized for its specific cleavage pattern.
B: Hind III, commonly used for its clean cuts in DNA.
C: Pst I, another precise cutter in genetic engineering tasks.
These sites are strategically used to insert or remove DNA fragments for cloning purposes.