Question

Hybridoma technique is used to obtain

• A. purified antibodies
• B. purified antigens
• C. purified lymphocytes
• D. purified macrophages

Hint:

Hybridoma = B lymphocyte plus myeloma cell, made to mass produce one specific antibody.

Answer 1

The Correct Option is A

Step 1: Understanding the Question.
We need to know what the hybridoma technique actually produces.

Step 2: Key Concept.
The hybridoma technique fuses an antibody producing B lymphocyte with an immortal myeloma (cancer) cell. The B lymphocyte gives the ability to make a specific antibody, and the myeloma cell gives the ability to divide endlessly in culture. The fused cell, called a hybridoma, keeps both properties.

Step 3: What comes out of the process.
Because each starting B lymphocyte makes one specific antibody, a single hybridoma clone keeps making that same, single, pure antibody in unlimited amounts. This is exactly how monoclonal antibodies, meaning pure antibodies of one specificity, are manufactured for research, diagnosis and treatment.

Step 4: Why the other options are wrong.
Purified antigens are the substances used to trigger an immune response in the first place, not the output of hybridoma technology. Purified lymphocytes and purified macrophages are cell types, and while a lymphocyte is used as one of the starting materials in making a hybridoma, the whole point of the technique is not to isolate more lymphocytes or macrophages, it is to get a steady supply of one pure antibody.

Step 5: Final Answer.
The hybridoma technique is used to obtain
\[ \boxed{\text{purified (monoclonal) antibodies}} \]