Question:
High molecular mass determined by which chromatographic technique:
High molecular mass determined by which chromatographic technique:
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Gel = Size; used for "big" molecules like proteins.
Updated On: May 25, 2026
- Partition chromatography
- Adsorption chromatography
- Ion-exchange chromatography
- Gel chromatography
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The Correct Option is D
Solution and Explanation
Step 1: Concept
Different chromatographic techniques separate molecules based on different physical or chemical properties.
Step 2: Meaning
Gel chromatography (also known as Size Exclusion Chromatography or Gel Filtration) uses a porous gel matrix to separate molecules.
Step 3: Analysis
In this method, molecules are separated strictly based on their size (hydrodynamic volume). Smaller molecules enter the pores and take longer to elute, while large molecules (high molecular mass) are excluded and elute first.
Step 4: Conclusion
Gel chromatography is the standard technique for determining the molecular weight distribution of polymers and proteins. Final Answer: (D)
Different chromatographic techniques separate molecules based on different physical or chemical properties.
Step 2: Meaning
Gel chromatography (also known as Size Exclusion Chromatography or Gel Filtration) uses a porous gel matrix to separate molecules.
Step 3: Analysis
In this method, molecules are separated strictly based on their size (hydrodynamic volume). Smaller molecules enter the pores and take longer to elute, while large molecules (high molecular mass) are excluded and elute first.
Step 4: Conclusion
Gel chromatography is the standard technique for determining the molecular weight distribution of polymers and proteins. Final Answer: (D)
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