Assertion A is true because ATP is indeed used at two steps in glycolysis. These steps are the conversion of glucose to glucose-6-phosphate, and the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate. In both of these steps, a phosphate group is added to the substrate, and ATP is used to provide the phosphate group.
Reason R is also true and provides the correct explanation for Assertion A. The first ATP is used in the conversion of glucose to glucose-6-phosphate, and the second ATP is used in the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate. Therefore, Reason R correctly explains why ATP is used at two steps in glycolysis.
Therefore, The correct answer is (D): Both A and R are true and R is the correct explanation of A.

Given below are two statements:
Statement I: Transfer RNAs and ribosomal RNA do not interact with mRNA.
Statement II: RNA interference (RNAi) takes place in all eukaryotic organisms as a method of cellular defence.
In the light of the above statements, choose the most appropriate answer from the options given below:
It is the process in which glucose is broken down to produce energy. It produces two molecules of pyruvate, ATP, NADH and water. If the process takes place in cytoplasm of a cell and it does not require oxygen. It occurs in both aerobic and anaerobic organisms.
