When a timeout occurs, TCP congestion control reduces the congestion window to \(1 \, \text{MSS}\) and enters the slow-start phase. In slow start:
During the first RTT, the window size doubles: \(1 \to 2 \, \text{MSS}\).
During the second RTT, the window size doubles again: \(2 \to 4 \, \text{MSS}\).
Thus, at the start of the third RTT, the congestion window size will be \(4 \, \text{MSS}\).
Final Answer:
\[
\boxed{4 \, \text{MSS}}
\]