Question

Choose the correct statements:
A. Tetrazolium test (TZ) is quick estimate of viability
B. In dormant seed tetrazolium test is not useful
C. Red colour parts of seed is related with living part
D. Generally, TZ test is correlated with germination test
E. In TZ test, dicot seed is damaged and could not be germinated

• A. A, B, C, D only
• B. A, B, E only
• C. A, C, D only
• D. B, C, E only

Hint:

TZ test is called a rapid viability test because it gives results within a few hours compared to several days required for germination tests.

Answer 1

The Correct Option is C

Concept: Tetrazolium test (TZ test) is a biochemical test used to determine seed viability rapidly by detecting respiration activity in living tissues.

Step 1: Understand the principle of TZ test.
Living cells contain active dehydrogenase enzymes that reduce tetrazolium salt into a red-colored compound called formazan. Therefore, red staining indicates living tissue, making statements A and C correct.

Step 2: Evaluate the remaining statements.
The TZ test is especially useful for dormant seeds because germination tests may fail to distinguish dormant seeds from dead seeds. TZ test results are generally correlated with germination tests. Thus, statement B is incorrect, while statement D is correct. Hence, the correct statements are A, C, and D only.