Question

Briefly explain the different steps involved in the construction of recombinant DNA.

Hint:

Steps in rDNA: Isolation → Cutting → Insertion → Transformation → Selection.
 

Answer 1

Recombinant DNA (rDNA) construction involves combining DNA from different sources to create new genetic combinations.

The key steps involved in recombinant DNA technology include:

• Isolation of DNA: Extracting the desired gene from the donor organism.
• Cutting of DNA: Using restriction enzymes to cut DNA at specific sites.
• Insertion into a vector: Ligating the gene into a vector such as a plasmid.
• Transformation: Introducing the recombinant DNA into a host cell.
• Selection: Identifying cells that have successfully taken up the recombinant DNA.