Biolistics method is suitable for gene transfer into ______.
- Viruses
- Animal cells
- Bacteria
- Plant cells
The Correct Option is D
Approach Solution - 1
DNA-coated metal particles (gold/tungsten) are accelerated into target cells using high pressure. Particles penetrate cell walls and membranes, delivering foreign DNA
Primary Application:
- Particularly effective for plant cells due to their rigid cell walls
- Widely used in crop genetic engineering (e.g., Bt cotton, Golden Rice)
Thus, the correct option is D: Plant cells. This method revolutionized plant genetic engineering by enabling direct gene transfer into intact plant tissues.
Approach Solution -2
Biolistics (gene gun): Fires gold/tungsten particles coated with DNA into plant cells (e.g., crops).
Biolistics (also known as the gene gun method) is a technique used to introduce foreign genetic material (DNA) into cells, particularly plant and animal cells, by physically shooting microscopic particles coated with DNA into the target cells. This method is particularly useful in genetic engineering, where researchers want to insert new genes into organisms.
Not suitable for:
- (A) Viruses: Too small.
- (B) Animal cells: Prefer electroporation/lipofection.
- (C) Bacteria: Use heat shock/electroporation.
Correct Answer: (D) Plant cells
Top KCET Biology Questions
- Relate Column I with Column II with regard to predatory behaviour:
Column I Column II 1. Calotropis p. Invertebrates 2. Pisaster q. Distasteful 3. Monarch butterfly r. Cryptically colored 4. Frogs s. Cardioglycoside - Small mammals and birds are rarely found in polar regions. The reason is that
Match Column I and Column I
Column I Column II 1 Narrowly utilitarian argument p Conserving biodiversity for major ecosystem services 2 Broadly utilitarian argument q Every species has an intrinsic value and moral duty to pass our biological legacy in good order to future generation. 3 Ethical argument r Receiving benefits like food, medicine & industrial products. - Identify the correct statement/s about ex situ conservation. Advanced ex situ conservation includes i. Cryopreservation of gametes. ii. Plant tissue culture method. iii. Seed bank. iv. In vitro fertilisation.
- Identify the odd one out.
Top KCET Genetics Questions
- Match Column I with Column II and find the correct answer :
Column I Column II 1. Aneuploidy p. Increase in whole set of chromosomes 2. Monoploidy q. Loss or gain of a chromosome 3. Polyploidy r. Two sets of chromosomes 4. Diploidy s. A single set of chromosomes - The affected male in the pedigreechart is symbolized by:
- Following representations P, Q, and R denote few steps of Griffith Experiment. Identify the correct one(s).
P. R strain → Inject into mice → Mice die
Q. S strain (Heat killed) → Inject into mice → Mice die
R. R strain → Inject into mice → Mice live - In tRNA, the region that binds with mRNA is:
- The mRNA has Untranslated Regions (UTRs):
Top KCET Questions
- Which of the following is not true for oxidation?
- If $a, b$ and $c \in N$ which one of the following is not true ?
- When $50\, cm ^{3}$ of $0.2\, N$ $H_{2} SO _{4}$ is mixed with $50 cm ^{3}$ of $1 N\, KOH$, the heat liberated is :
- If $a | (b + c)$ and $a| (b-c)$ where $a,b,c \in \, N $ then
- Which of the following is NOT a pair of functional isomers?