The steps of PCR are as follows:
1. Denaturation: Heat the double-stranded DNA to separate it into single strands.
2. Annealing: Lower the temperature to allow primers to bind to complementary sequences.
3. Extension: DNA polymerase extends the primers by synthesizing a new strand of DNA.
4. Cycling: These steps are repeated multiple times to amplify the target DNA sequence.
| List I (Enzyme) | List II (Function) |
|---|---|
| (A) Cytochrome oxidase | (I) Electron transport system |
| (B) Topoisomerases | (II) Change linking number |
| (C) Cohesins | (III) DNA Replication |
| (D) PCNA | (IV) Cell cycle |