Question

Arrange the following processes of recombinant DNA Technology in a correct sequence: A. Amplification of Gene of Interest using PCR B. Isolation of genetic material (DNA) C. Obtaining foreign gene product D. Insertion of Recombinant DNA into host cell/organism E. Cutting of DNA at specific locations

• A. C, A, B, D, E
• B. B, E, A, D, C
• C. A, D, B, C, E
• D. D, B, C, E, A

Hint:

rDNA steps: Isolate $\rightarrow$ Cut $\rightarrow$ Amplify $\rightarrow$ Insert into host $\rightarrow$ Express product.

Answer 1

The Correct Option is B

Step 1: Concept
Sequential steps in recombinant DNA technology.

Step 2: Meaning
Recombinant DNA technology involves several ordered steps from DNA isolation to product expression.

Step 3: Analysis
B: Isolate source DNA.
E: Cut DNA at specific locations using restriction endonucleases.
A: Amplify the gene of interest by PCR.
D: Insert recombinant DNA into host cell (transformation/transfection).
C: Express gene and obtain the foreign gene product.

Step 4: Conclusion
Correct sequence: B $\rightarrow$ E $\rightarrow$ A $\rightarrow$ D $\rightarrow$ C. Final Answer: (B)