Question:
Arrange the following components of a high performance liquid chromatography (HPLC) instrument in the correct order of the mobile phase flow path.
A. Pumping system
B. Injection valve
C. Detector
D. Column
Choose the correct answer from the options given below :
Arrange the following components of a high performance liquid chromatography (HPLC) instrument in the correct order of the mobile phase flow path.
A. Pumping system
B. Injection valve
C. Detector
D. Column
Choose the correct answer from the options given below :
B. Injection valve
C. Detector
D. Column
Choose the correct answer from the options given below :
Show Hint
HPLC flow: Pump → Injector → Column → Detector.
Updated On: May 18, 2026
- A, D, B, C
- D, A, B, C
- B, A, D, C
- A, B, D, C
Show Solution
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The Correct Option is D
Solution and Explanation
Concept:
In HPLC, the mobile phase flows through various components in a defined sequence, ensuring proper sample introduction, separation, and detection.
Step 1: Pumping system (A).
The pump is responsible for delivering the mobile phase at high pressure through the system.
Step 2: Injection valve (B).
The sample is introduced into the mobile phase stream through the injection valve.
Step 3: Column (D).
The mixture passes through the column where separation of components occurs based on interactions with the stationary phase.
Step 4: Detector (C).
Finally, separated components are detected and recorded.
Step 5: Forming correct sequence.
\[ A \rightarrow B \rightarrow D \rightarrow C \] Final Conclusion:
Hence, the correct answer is option (4).
Step 1: Pumping system (A).
The pump is responsible for delivering the mobile phase at high pressure through the system.
Step 2: Injection valve (B).
The sample is introduced into the mobile phase stream through the injection valve.
Step 3: Column (D).
The mixture passes through the column where separation of components occurs based on interactions with the stationary phase.
Step 4: Detector (C).
Finally, separated components are detected and recorded.
Step 5: Forming correct sequence.
\[ A \rightarrow B \rightarrow D \rightarrow C \] Final Conclusion:
Hence, the correct answer is option (4).
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