List of top Biochemistry Questions asked in GATE BT

An enzyme-catalyzed reaction is found to have \(\Delta G = -100\) kJ mol\(^{-1}\). Which of the following statements about this reaction is/are true?

You have purified an enzyme using a series of chromatographic methods. It was observed that a 10 \(\mu\)g mL^-1 of this purified enzyme converted 10 mM substrate per hour at 25\(^{\circ}\)C and pH 7. Its specific activity is IU \(\mu\)g^-1. (rounded off to three decimal places)

Which of the following enzyme is involved in the protection of cells from reactive oxygen species?

An enzyme (E) catalyzes the biochemical reaction \(A \rightarrow B\) with \(k_{\text{cat}}=500~\text{s}^{-1}\). If the initial reaction velocity \((V_0)\) is \(10~\mu\text{M}\cdot\text{s}^{-1}\) at the total enzyme concentration \([E_t]=30~\text{nM}\) and substrate concentration \([A]=40~\mu\text{M}\), the value of \(K_m\) (in \(\mu\text{M}\)) is ________________.

The number of different possible ways of forming five intramolecular disulfide bonds with ten cysteine residues of a protein is ________________.

Intracellular proteins are targeted for proteolytic degradation in proteasomes upon conjugation with