Question

With help of diagram, show the steps of recombinant DNA technology.

Hint:

Restriction enzymes are called "molecular scissors" because they cut DNA at specific palindromic sequences.

Answer 1

Step 1: The DNA of interest and a vector (plasmid) are cut using the same restriction enzyme to create compatible "sticky ends."
Step 2: The gene of interest is joined to the vector using the enzyme DNA Ligase, forming Recombinant DNA (rDNA).
Step 3: The rDNA is introduced into a host cell (like E. coli) through transformation. The host cells are then cultured at a large scale to produce the desired protein.