Question:
Which of the following technique(s) can be used to separate proteins according to their molecular weights from a mixture of proteins?
Which of the following technique(s) can be used to separate proteins according to their molecular weights from a mixture of proteins?
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Always link the separation principle to the property: charge (ion exchange), size/shape (size exclusion), molecular weight (SDS–PAGE), or density/size (gradient centrifugation).
Updated On: Sep 1, 2025
- Ion exchange chromatography
- Size exclusion chromatography
- Sodium dodecylsulfate–polyacrylamide gel electrophoresis (SDS–PAGE)
- Sucrose density gradient centrifugation
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The Correct Option is B, C, D
Solution and Explanation
(A) Ion exchange chromatography separates proteins based on their charge, not molecular weight. ❌
(B) Size exclusion chromatography separates proteins based on size/shape, effectively correlating with molecular weight. ✔️
(C) SDS–PAGE separates denatured proteins by molecular weight after SDS treatment equalizes charge-to-mass ratio. ✔️
(D) Sucrose density gradient centrifugation separates macromolecules based on sedimentation rate, which depends largely on size and mass. ✔️
Hence, correct techniques are (B), (C), and (D).
(B) Size exclusion chromatography separates proteins based on size/shape, effectively correlating with molecular weight. ✔️
(C) SDS–PAGE separates denatured proteins by molecular weight after SDS treatment equalizes charge-to-mass ratio. ✔️
(D) Sucrose density gradient centrifugation separates macromolecules based on sedimentation rate, which depends largely on size and mass. ✔️
Hence, correct techniques are (B), (C), and (D).
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