Which of the following is a restriction endonuclease ?
- Protease
- DNase I
- RNase I
- Hind II
The Correct Option is D
Solution and Explanation
The correct answer is:
Option 4: Hind II
Explanation:
Protease: Proteases are enzymes that break down proteins by hydrolyzing peptide bonds. They are not restriction endonucleases.
DNase I: DNase I is an enzyme that cleaves DNA into smaller fragments but is not classified as a restriction endonuclease. It is a general endonuclease that cuts at random locations in DNA.
RNase I: RNase I is an enzyme that degrades RNA molecules, not DNA. It is not a restriction endonuclease.
Hind II: Hind II is a restriction endonuclease. It is an enzyme that cuts DNA at specific recognition sites. Restriction endonucleases, like Hind II, recognize a specific sequence of nucleotides in a DNA molecule and cut it at or near that site.
Thus, Hind II is the correct answer as it is a restriction endonuclease.
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Concepts Used:
Biotechnology
Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."
Principles of Biotechnology:
Among many, the two core techniques that entitled the birth of modern biotechnology are :
- Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
- Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.