What does the sample of given base sequence represent? 5' - GAATTC - 3' 3' - CTTAAG - 5'
- Palindromic sequence
- Initiator codon at 5' end
- Deletion mutation
- Completion of replication
The Correct Option is A
Approach Solution - 1
A palindromic sequence is a DNA sequence that reads the same on both strands when read in the 5' to 3' direction. In this case, the sequence "GAATTC" on one strand is complementary to the sequence "CTTAAG" on the opposite strand.
The recognition of palindromic sequences by specific restriction enzymes is important in molecular biology. Many restriction enzymes recognize and cleave DNA at specific palindromic sequences. In this example, the palindromic sequence "GAATTC" is recognized by the restriction enzyme EcoRI, which cleaves the DNA at that specific site.
Therefore, the given base sequence represents a palindromic sequence, where the sequence on one strand is the reverse complement of the sequence on the other strand.
Approach Solution -2
The given base sequence:
5' - GAATTC - 3'
3' - CTTAAG - 5'
represents the recognition site for the restriction enzyme EcoRI.
Explanation:
EcoRI is a restriction endonuclease that recognizes the palindromic sequence 5'-GAATTC-3' and cuts between G and A, producing sticky ends.
This enzyme is widely used in genetic engineering and recombinant DNA technology for cutting and inserting DNA fragments.
Thus, the given sequence represents a restriction site for EcoRI.
Top KCET Biology Questions
- Relate Column I with Column II with regard to predatory behaviour:
Column I Column II 1. Calotropis p. Invertebrates 2. Pisaster q. Distasteful 3. Monarch butterfly r. Cryptically colored 4. Frogs s. Cardioglycoside - Small mammals and birds are rarely found in polar regions. The reason is that
Match Column I and Column I
Column I Column II 1 Narrowly utilitarian argument p Conserving biodiversity for major ecosystem services 2 Broadly utilitarian argument q Every species has an intrinsic value and moral duty to pass our biological legacy in good order to future generation. 3 Ethical argument r Receiving benefits like food, medicine & industrial products. - Identify the correct statement/s about ex situ conservation. Advanced ex situ conservation includes i. Cryopreservation of gametes. ii. Plant tissue culture method. iii. Seed bank. iv. In vitro fertilisation.
- Identify the odd one out.
Top KCET Structure of DNA and RNA Questions
- The codons UUU and UUC codes for phenylalanine only. This feature of genetic code is called
- RNA polymerase-I transcribes eukaryotic ribosome which does not consist of
- Which of these is NOT a method to make host cells ' competent ' to take up DNA ?
- In Structural gene, the template DNA strand has nucleotide sequences
3' ATGCATGCATGCATGC 5'.
Find the correct and complimentary nucleotide sequence on coding strand.
Top KCET Questions
- Which of the following is not true for oxidation?
- If $a, b$ and $c \in N$ which one of the following is not true ?
- When $50\, cm ^{3}$ of $0.2\, N$ $H_{2} SO _{4}$ is mixed with $50 cm ^{3}$ of $1 N\, KOH$, the heat liberated is :
- If $a | (b + c)$ and $a| (b-c)$ where $a,b,c \in \, N $ then
- Which of the following is NOT a pair of functional isomers?