Question

To prevent contamination during setup of PCR reaction, which one of these is not important?

• A. Pre- and post-PCR samples should be processed in separate areas
• B. Equipment used for pre- and post-PCR steps should be cleaned after every use
• C. Reagents used for pre- and post-PCR steps should be separated
• D. Adding smaller amounts of Bovine Serum Albumin (BSA) is avoided

Hint:

Focus on physical barriers, proper equipment cleaning, and reagent management to maintain a contamination-free PCR setup.

Answer 1

The Correct Option is D

BSA is often added to PCR reactions to stabilize enzymes and improve efficiency, but it does not prevent contamination. Preventing contamination requires physical separation of pre- and post-PCR areas, cleaning equipment, and separating reagents. BSA is unrelated to contamination control.