The number of peptide fragments generated from the sequence: Ala-Trp-Val-Ala-Phe-Thr-Gly-Lys-Glu-Tyr-Asp-Ser-Lys on treatment with chymotrypsin is ..........
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Correct Answer: 3.9
Solution and Explanation
Step 1: Understand the specificity of chymotrypsin.
Chymotrypsin is a protease that cleaves peptide bonds on the carboxyl side of aromatic amino acids, such as phenylalanine (Phe), tyrosine (Tyr), and tryptophan (Trp).
Step 2: Identify the cleavage sites.
In the given sequence, chymotrypsin will cleave after:
- Phe (position 5)
- Trp (position 2)
- Tyr (position 9)
The sequence contains 3 cleavage sites, resulting in 4 fragments.
Step 3: Conclusion.
The number of peptide fragments generated after treatment with chymotrypsin is 4.
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