The weight of agarose required is calculated as:
\( \text{Weight} = \text{Concentration} \times \text{Volume}. \)
Substitute 0.8% (0.8 g/100 mL) and 250 mL:
\( \text{Weight} = 0.8 \times \frac{250}{100} = 2 \text{ g}. \)
| Group I | Group II |
| P) NaCl | 1) Coordination bond |
| Q) $H_2$ | 2) Polar covalent bond |
| R) $Pd-P$ bond in $Pd(PPh_3)_4 | 3) Covalent bond |
| S) $C-Cl$ bond in $CH_3Cl $ | 4) Ionic bond |

